The most important improvement that results from the application of ISFETs instead of glass membrane electrodes is in the method of fabrication. This paper reviews the results that have been reported on ISFET based enzyme sensors. This presentation will focus on calibration of the ISFET pH sensor, evaluate its analytical performance, and validate performance using recent field data. Sea-Bird Scientific continues to advance this technology through a concerted effort to improve pH sensor accuracy and reliability by characterizing their performance in the laboratory and field. Sea-Bird Scientific played a critical role in the adoption this new technology by commercializing the SeaFET pH sensor and float pH Sensor developed by the MBARI chemical sensor group. However, with the introduction of robust and stable ISFET pH sensors for use in ocean applications a paradigm shift in the methods used to acquire long-term pH time series data has occurred. Historically, ocean pH time series data was acquired from infrequent shipboard grab samples and measured using labor intensive spectrophotometry methods. Field Performance of ISFET based Deep Ocean pH Sensors
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